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Silicon Lab-on-a-chip Test Could Provide Cheaper, Portable Diagnostics

Credit: Imperial College London

Known as TriSilix, a new ‘micro laboratory’ developed by scientists at Imperial College London performs a miniature version of the polymerase chain reaction (PCR) on the spot to detect viruses and bacteria in a matter of minutes.

The chip is made from silicon, the same material that is used to make electronic chips. Silicon itself is cheap, however, it is expensive to process into chips which requires massive, ‘extremely clean’ factories otherwise known as cleanrooms. To make the new lab-on-chip, the researchers developed a series of methods to produce the chips in a standard laboratory, cutting the costs and time they take to fabricate, potentially allowing them to be produced anywhere in the world.

“Rather than sending swabs to the lab or going to a clinic, the lab could come to you on a fingernail-sized chip,” explained Lead researcher Dr. Firat Guder of Imperial’s Department of Bioengineering. “You would use the test much like how people with diabetes use blood sugar tests, by providing a sample and waiting for results – except this time it’s for infectious diseases.”

The researchers have so far used TriSilix to diagnose a bacterial infection mainly present in animals as well as a synthetic version of the genetic material from SARS-CoV-2, the virus behind COVID-19.

The team say the system could in future be mounted onto handheld blood sugar test-style devices. This would let people test themselves and receive results at home for colds, flu, recurrent infections like those of the urinary tract (UTIs), and COVID-19.

Table-top devices for testing of infections like COVID-19 already exist, but these tests can be time-consuming and costly since the patient must go to a clinic, have a sample taken by a healthcare worker and go home or stay in clinic to wait. People leaving their homes when not feeling well increases the risk of spread of a pathogen to others.

If validated on human samples, this new test could provide results outside a clinic, at home or on-the-go within minutes.

Making testing more accessible and cheaper is especially important for people in rural areas of low-income countries, where clinics can be far away and expensive to travel to.

Each lab-on-a-chip contains a DNA sensor, temperature detector and heater to automate the testing process. A typical smartphone battery could power up to 35 tests on a single charge.

Next, the researchers plan to validate their chip with clinical samples, automate the preparation of samples and advance their handheld electronics. They are looking for partners and funders to help accelerate the translation of the technology deliver testing at resource limited settings at homes, farms or remote locations in the developing world.

Disposable Silicon-based All-in-One Micro-qPCR for Rapid On-Site Detection of Pathogens by Firat Güder et al., published 2 December 2020 in Nature Communications

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