A new study from the University of Missouri and Colorado State University outlines the application of CRISPR gene-editing technology to produce mosquitoes that are unable to replicate Zika virus, rendering them unable to infect humans.
The Zika virus epidemic is a pressing public health emergency. In Brazil for example, there has been a 10-fold increase in newborns with microcephaly since October 2015, compared with previous years. Researchers have wrestled with various strategies for controlling the spread of Zika virus, which is transmitted to humans from female mosquito bites.
One approach, which was approved by the Environmental Protection Agency in May, will release more than 750 million genetically modified mosquitos into the Florida Keys in 2021 and 2022. These “suicide mosquitos” are genetically-altered to produce offspring that die before emerging into adults and therefore cannot bite humans and spread disease.
However, wiping out future generations of mosquitoes may cause environmental complications, such as potentially disrupting food chains. A new research study at the University of Missouri offers another option: genetically modifying mosquitoes to be resistant to Zika virus altogether.
Alexander Franz, an associate professor in the MU College of Veterinary Medicine, collaborated with researchers at Colorado State University by using CRISPR gene-editing technology to produce mosquitoes that are unable to replicate Zika virus and therefore cannot infect a human through biting.
“We genetically manipulated these mosquitoes by inserting an artificial gene into their genome that triggers one of the immune pathways in the midgut to recognize and destroy the RNA genome of Zika virus. By developing these mosquitoes that are resistant to the virus, the disease cycle is interrupted so transmission to humans can no longer take place.”Alexander Franz, MU College of Veterinary Medicine
Franz added that the genetic modification is inheritable, so future generations of the altered mosquitoes would be resistant to Zika virus as well.
“We are interested in strategies for controlling insect vectors like mosquitoes that transmit various viruses affecting human health,” Franz said. “Public health experts suggest having a toolbox with different approaches available to tackle a virus such as Zika, and unfortunately right now there are limited options. There is no vaccine for the Zika virus widely available and spraying insecticides has become ineffective since the mosquitoes can develop resistance, so we are simply trying to expand the toolbox and provide a solution by genetically modifying the mosquitoes to become Zika-resistant while keeping them alive at the same time.”
Franz’ research is designed to help prevent another outbreak of Zika virus disease from occurring.
“If you can ever find a way to block the transmission of a pathogen that negatively affects humans, that is good news,” Franz said. “We have shown this is a viable option for genetically modifying mosquitos in a lab setting. There would need to be thorough discussions about regulatory compliance to see if this can be a solution out in the field down the road, and who knows when another Zika outbreak might happen in the future, which is why this research is so important.”
The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti. Viruses, 30 October 2020.