This study has opened up new possibilities for surface plasmon resonance (SPR) screening of different monoclonal antibodies of Biological Warfare Agents (BWA).
Here, different monoclonal antibodies (mAb1, mAb2 and mAb3) of Ebola virus were screened in a real-time and label-free manner using SPR to select an appropriate antibody for biosensor applications against a biological warfare agent.
For this purpose, a gold SPR chip was modified with 4-mercaptobenzoic acid (4-MBA), and modification was confirmed by FTIR-ATR and EIS. The 4-MBA-modified gold SPR chip was used for immobilization of the recombinant nucleoprotein of Ebola (EBOV-rNP), and the interactions of mAb1, mAb2 and mAb3 were then investigated to determine the best mAb.
High affinity was shown for mAb3. This was confirmed by ELISA results. The thermodynamic parameters for the interaction between mAb3 and EBOV-rNP were also determined, which revealed that the interaction was spontaneous, endothermic and driven by entropy. The SPR limit of detection of EBOV-rNP with mAb3 was 0.5 pg ml−1, showing mAb3 to be the best high-affinity antibody in our study.
Surface plasmon resonance sensing of Ebola virus: a biological threat. Analytical and Bioanalytical Chemistry (2020). 19 April 2020.