Rubella virus, since it was first identified in 1962, has been the only known member of its virus family, Matonaviridae. That is, until two separate teams, working in completely different parts of the world, converged on discoveries that would forever link them.
In a Ugandan forest, a team of American and African scientists took oral swabs from insect-eating cyclops leaf-nosed bats and discovered a new relative of Rubella, now known as the ruhugu virus.
The virus found in Germany, slightly different from rubella and ruhugu, is rustrela, discovered in a Bennett’s tree-kangaroo and a capybara at a German zoo — which were suffering from severe brain swelling.
The two teams have now collaborated to publish their findings today in Nature. They describe the new viruses, their similarities to rubella virus, and their differences. Neither of the new viruses are known to infect people.
“Why has it been so challenging to track down the origins or relatives of rubella virus?” asks Tony Goldberg, a University of Wisconsin-Madison professor of epidemiology at the School of Veterinary Medicine, who led the American efforts. “Why did it take 206 years from the time George Maton first described rubella, and why did two teams working independently figure it out within three months of each other, get lucky enough to learn of one another’s results, and then lucky enough to work together to publish?”
It isn’t because people haven’t tried, Goldberg says. It may be that advancing technology has made it easier — rubella virus genomes are notoriously difficult to work with, and the new viruses share these characteristics.
It may just be serendipity.
Goldberg’s team — whose efforts in the new study were led by his former graduate student Andrew Bennett — wasn’t even looking for rubella-like viruses. They were, pre-COVID-19 pandemic, working with their Ugandan colleagues to look for coronaviruses carried by bats. Ruhugu virus popped up as a strange string of letters in the giant analyses of genetic code the team was performing on samples collected from the bats.
When they looked closer, they saw that it was quite similar to rubella virus, just one short word, or amino acid, different in a key region of the genome that lets viruses bind to host cells. (Rustrela virus has a few more amino acid differences.) The researchers are currently working to further study both viruses in the lab.
Rubella is an airborne virus that has largely been eradicated thanks to an effective vaccine, though pockets of disease still exist throughout the world. It can cause rash and flu-like symptoms. During pregnancy, the virus can cause miscarriage, stillbirth or fetal development defects — as many as 100,000 children each year are born with congenital rubella syndrome and may be deaf, blind or experience heart problems.
Rubella has not been found in animals, which has made it easier for the World Health Organization to target elimination of the virus. However, both of the new viruses have been found in common mammal species in Uganda and in Germany (researchers have found rustrela virus in yellow-necked field mice). Up to half of the bats and half of the mice tested were carriers of their respective viruses. This suggests both species may act as viral reservoirs, carrying and transmitting pathogens without getting sick.
The study also indicates that rubella, like many other human viruses, probably originated in animals. Researchers do not know whether rubella virus can jump back into animals.
“There is no evidence that ruhugu virus or rustrela virus can infect people, yet if they could, it might be so consequential that we should consider the possibility,” Goldberg says. “We know that in Germany, rustrela virus jumped among species that are not at all closely related. If either of these viruses turns out to be zoonotic, or if rubella virus can go back into animals, that would be a game changer for rubella eradication.”
The team’s analysis suggests the three viruses may be similar enough that the current rubella vaccine could be effective against all of them — a key question for research going forward, says Goldberg, also a member of the UW-Madison Global Health Institute.
The new viruses also provide scientists with new tools to probe the biology of rubella virus and the Matonaviridae family. There are no good animal models for rubella, but rustrela virus provides a new opportunity to explore one. Mice are common model species in the laboratory.
Additionally, the findings reinforce the critical importance of conservation efforts in Uganda to protect forests from encroaching development, and the important work scientists and others there are undertaking to study the effects of a changing environment on human and animal diseases.
For instance, Deputy Director of Field Operations for the Uganda Wildlife Authority Charles Tumwesigye says the study’s findings will be incorporated into UWA’s community conservation awareness programs, especially around Kibale National Park, where the ruhugu virus was found.
The study “will help management to further protect unique aspects of the ecosystem as well as keep the population safe,” he says. “Uganda Wildlife Authority values scientific research because it provides key information for decision-making in protected area management.”
Tumwesigye adds that the ways in which people and wildlife relate are “key to harmonious co-existence … When communities around Kibale National Park appreciate the value of the bats, for example, they become supportive in their conservation initiatives and learn how to protect themselves.”
If not for the country’s “long history of excellent medical and conservation science,” says Goldberg, “there might have been no bats to study.”
Protecting habitat is of utmost importance to animals and to people, he adds. “Viruses stay in their place when ecosystems are intact.”
Relatives of rubella virus in diverse mammals. Nature, October 7, 2020.