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Home Biodetection

Army Seeks Botulinum Neurotoxin Assays

by Global Biodefense Staff
April 23, 2014

The U.S. Army Medical Research Acquisition Activity (USAMRAA) is seeking assay kits for the routine detection of botulinum neurotoxin (BoNT) serotypes A B, C, D, E, F and G.

The assay must have the ability to measure accurately BoNT’s proteolytic cleavage of natural BoNT substrates – SNAP25 or VAMP2 – in a sensitive, FRET-based, mix and read format and using most standard fluorescent plate readers.

The FRET-based nature of the assays allows for real-time detection of BoNT proteolytic activity enabling the determination of kinetic constants and the determination of enzymatic activity. The assay must have picomolar to fentomolar sensitivity.

Further specifications are detailed under Solicitation Number: W81XWH14T0160.

Source: FBO.gov

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